goat anti cd31 (R&D Systems)
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Goat Anti Cd31, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+cd31/Human%2FMouse%2FRat+CD31%2FPECAM-1+Antibody/pmc13061539-116-12-16
Average 98 stars, based on 1263 article reviews
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Immunostaining:Article Title: Galectin-3 mediates lysosome-related inflammation within monocyte-derived macrophages in a mouse model of ischemic brain injury Article Snippet: .. For immunostaining, we used goat anti-IBA1 (Abcam, ab5076), rabbit anti-NLRP3 (Abcam, ab263899), rabbit anti-CD11c (Abcam, ab219799), rat anti-GFAP (Invitrogen, 13-0300), rat anti-CD68 (Invitrogen, 14-0681-82), Article Title: Impaired vitamin D signaling reveals neutrophils as key drivers of prostate cancer dissemination. Article Snippet: .. Immunostaining was performed by an overnight incubation at 4 °C with primary antibodies [rabbit antip-Akt Ser473 (Cell Signaling Technology Cat# 4060, RRID:AB_2315049; 1:200 dilution), rabbit anti-α-SMA (Cell Signaling Technology Cat# 19245, RRID:AB_2734735; 1:200 dilution), mouse anti-8-OHdG (Abcam Cat# ab48508, RRID:AB_867461; 1:100 dilution), rabbit anti-Ki-67 (Thermo Fisher Scientific Cat# MA5-14520, RRID:AB_10979488; 1:200 dilution), rabbit antiPanCK (Proteintech Cat# 26411-1-AP, RRID:AB_2880505; 1:500 dilution), rabbit anti-CD45 (Abcam Cat# ab10558, RRID:AB_442810; 1:600 dilution), Article Title: Galectin-3 mediates lysosome-related inflammation within monocyte-derived macrophages in a mouse model of ischemic brain injury Article Snippet: .. For immunostaining: goat anti-IBA1 (Abcam, ab5076), rabbit anti-NLRP3 (Abcam, ab263899), rabbit anti-CD11c (Abcam, ab219799), rat anti-GFAP (Invitrogen, 13-0300), rat anti-CD68 (Invitrogen, 14-0681-82), Incubation:Article Title: Impaired vitamin D signaling reveals neutrophils as key drivers of prostate cancer dissemination. Article Snippet: .. Immunostaining was performed by an overnight incubation at 4 °C with primary antibodies [rabbit antip-Akt Ser473 (Cell Signaling Technology Cat# 4060, RRID:AB_2315049; 1:200 dilution), rabbit anti-α-SMA (Cell Signaling Technology Cat# 19245, RRID:AB_2734735; 1:200 dilution), mouse anti-8-OHdG (Abcam Cat# ab48508, RRID:AB_867461; 1:100 dilution), rabbit anti-Ki-67 (Thermo Fisher Scientific Cat# MA5-14520, RRID:AB_10979488; 1:200 dilution), rabbit antiPanCK (Proteintech Cat# 26411-1-AP, RRID:AB_2880505; 1:500 dilution), rabbit anti-CD45 (Abcam Cat# ab10558, RRID:AB_442810; 1:600 dilution), Article Title: Neutrophil Extracellular Traps Promote AIM2-Dependent Microglial Pyroptosis Following Stroke Article Snippet: .. Tissue sections were incubated with primary antibodies targeting key cellular markers, including Article Title: In vivo discovery of blood-brain barrier opening small molecules with FishNAP Article Snippet: .. Sagittal sections were blocked for 1 h at room temperature in PBS containing 10% normal donkey serum and 0.1% Triton X-100, then incubated overnight at 4°C with primary antibodies diluted in blocking buffer: Microscopy:Article Title: Metabolite-induced DNA damage drives stochastic stem cell loss and clonal hematopoiesis Article Snippet: Cell suspensions from either source were spun unto slides using a Cytospin 4 (Thermo Scientific) and then stained using modified Wright stain (06689653 [Hematek Stain Pak] Hematek) on an automated staining platform (Hematek Bayer Health Care). .. Embryo whole-mount microscopy and analysis Whole-mount immunofluorescence on embryos was performed as previously described.38,96 Specifically, developmentally equivalent embryos (as assessed by somite count and vessel architecture) were stained using Immunofluorescence:Article Title: Metabolite-induced DNA damage drives stochastic stem cell loss and clonal hematopoiesis Article Snippet: Cell suspensions from either source were spun unto slides using a Cytospin 4 (Thermo Scientific) and then stained using modified Wright stain (06689653 [Hematek Stain Pak] Hematek) on an automated staining platform (Hematek Bayer Health Care). .. Embryo whole-mount microscopy and analysis Whole-mount immunofluorescence on embryos was performed as previously described.38,96 Specifically, developmentally equivalent embryos (as assessed by somite count and vessel architecture) were stained using Staining:Article Title: Metabolite-induced DNA damage drives stochastic stem cell loss and clonal hematopoiesis Article Snippet: Cell suspensions from either source were spun unto slides using a Cytospin 4 (Thermo Scientific) and then stained using modified Wright stain (06689653 [Hematek Stain Pak] Hematek) on an automated staining platform (Hematek Bayer Health Care). .. Embryo whole-mount microscopy and analysis Whole-mount immunofluorescence on embryos was performed as previously described.38,96 Specifically, developmentally equivalent embryos (as assessed by somite count and vessel architecture) were stained using Blocking Assay:Article Title: In vivo discovery of blood-brain barrier opening small molecules with FishNAP Article Snippet: .. Sagittal sections were blocked for 1 h at room temperature in PBS containing 10% normal donkey serum and 0.1% Triton X-100, then incubated overnight at 4°C with primary antibodies diluted in blocking buffer: |
![Superior SA node myocytes exhibit elevated diastolic ATP and metabolic flux compared with the inferior region. (A) 3D segmented maximum-intensity projection of a whole-mount SA node immunolabeled for <t>CD31</t> (vasculature, red) and cyto-iATP (myocytes, green). The dashed line denotes the boundary between superior and inferior regions. (B) Image-processing workflow illustrating merged maximum-intensity projections, binary segmentation masks, and extraction of grayscale cyto-iATP signals used for quantitative analysis. (C) Mean cyto-iATP fluorescence intensity per myocyte, grouped by region ( N = 5 mice per region), reporting expression levels of the EGFP-tagged cyto-iATP sensor. (D) Live confocal imaging of cyto-iATP signals showing representative line-scan images and corresponding normalized fluorescence traces (F/F 0 ) from superior and inferior regions. (E and F) Summary quantification of cyto-iATP signal mass rate (E) and estimated diastolic [ATP] i (F). P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_7587/pmc13037587/pmc13037587__jgp_202513874_fig1.jpg)