goat anti mouse cd31 (R&D Systems)
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goat anti mouse cd31
![a – c Mice were fed with a standard or CDAA diet for 10 weeks. a Images show Picrosirius Red staining on the liver sections. LSECs were isolated from healthy and fibrotic liver tissues. Scale bar = 100 µm. b The dot plot shows the number of LSECs isolated from fibrotic mouse liver. [mean ± SD, n = 4 mice]. c Quantitative PCR analyses to compare the expression level of various sinusoidal genes in LSECs isolated from either healthy or fibrotic mouse liver tissues. [mean ± SD, n = 4 mice]. * P < 0.05 (Mann–Whitney test). d – f Liver tissues collected from healthy pigs were processed to isolate LSECs. d FACS plot showing gating strategy to isolate <t>CD31+</t> LSECs and CD31− cells. e The dot plot shows the number of LSECs isolated from pig liver tissues. [mean ± SD, n = 4 pigs]. f Quantitative PCR analyses to compare expression of various vascular genes between CD31+ LSECs and CD31− cells. [mean ± SD, n = 4 pigs]. * P < 0.05 (Mann–Whitney test).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1496/pmc11711496/pmc11711496__42003_2025_7458_Fig3_HTML.jpg)
Goat Anti Mouse Cd31, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1010 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti mouse cd31/product/R&D Systems
Average 96 stars, based on 1010 article reviews
Goat Anti Mouse Cd31, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1010 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti mouse cd31/product/R&D Systems
Average 96 stars, based on 1010 article reviews
goat anti mouse cd31 - by Bioz Stars,
2026-03
96/100 stars
Images
1) Product Images from "A perfusion-independent high-throughput method to isolate liver sinusoidal endothelial cells"
Article Title: A perfusion-independent high-throughput method to isolate liver sinusoidal endothelial cells
Journal: Communications Biology
doi: 10.1038/s42003-025-07458-5
Figure Legend Snippet: a – c Mice were fed with a standard or CDAA diet for 10 weeks. a Images show Picrosirius Red staining on the liver sections. LSECs were isolated from healthy and fibrotic liver tissues. Scale bar = 100 µm. b The dot plot shows the number of LSECs isolated from fibrotic mouse liver. [mean ± SD, n = 4 mice]. c Quantitative PCR analyses to compare the expression level of various sinusoidal genes in LSECs isolated from either healthy or fibrotic mouse liver tissues. [mean ± SD, n = 4 mice]. * P < 0.05 (Mann–Whitney test). d – f Liver tissues collected from healthy pigs were processed to isolate LSECs. d FACS plot showing gating strategy to isolate CD31+ LSECs and CD31− cells. e The dot plot shows the number of LSECs isolated from pig liver tissues. [mean ± SD, n = 4 pigs]. f Quantitative PCR analyses to compare expression of various vascular genes between CD31+ LSECs and CD31− cells. [mean ± SD, n = 4 pigs]. * P < 0.05 (Mann–Whitney test).
Techniques Used: Staining, Isolation, Real-time Polymerase Chain Reaction, Expressing, MANN-WHITNEY
![a – d LSECs were isolated using perfusion independent digestion method, followed by positive selection using CD146-magnetic beads. After that, enriched LSECs were placed in culture. a Primary LSEC cultures were stained for an endothelial cell marker (ERG, in green) and nuclear marker (DAPI, in gray). On the left, images show IF-stained LSEC cultures. Scale bar = 25 µm. The yellow arrow highlights an ERG-negative cell. On the right, the dot plot shows the percentage of ERG-positive cells of DAPI-positive cells. [mean ± SD, n = 3 wells]. b Images show IF-stained LSECs with CD31 (in gray), Stabilin-2 (in green), and DAPI (in blue). Scale bar = 25 µm. c Images show IF-stained LSECs with CD32b (in gray) and ERG (in green). Scale bar = 25 µm. d Representative scanning electron microscopy images of cultured LSECs. Scale bar = 2 µm.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1496/pmc11711496/pmc11711496__42003_2025_7458_Fig4_HTML.jpg "... 3 wells]. b Images show IF-stained LSECs with CD31 (in gray), Stabilin-2 (in green), and DAPI (in ...")
Figure Legend Snippet: a – d LSECs were isolated using perfusion independent digestion method, followed by positive selection using CD146-magnetic beads. After that, enriched LSECs were placed in culture. a Primary LSEC cultures were stained for an endothelial cell marker (ERG, in green) and nuclear marker (DAPI, in gray). On the left, images show IF-stained LSEC cultures. Scale bar = 25 µm. The yellow arrow highlights an ERG-negative cell. On the right, the dot plot shows the percentage of ERG-positive cells of DAPI-positive cells. [mean ± SD, n = 3 wells]. b Images show IF-stained LSECs with CD31 (in gray), Stabilin-2 (in green), and DAPI (in blue). Scale bar = 25 µm. c Images show IF-stained LSECs with CD32b (in gray) and ERG (in green). Scale bar = 25 µm. d Representative scanning electron microscopy images of cultured LSECs. Scale bar = 2 µm.
Techniques Used: Isolation, Selection, Magnetic Beads, Staining, Marker, Electron Microscopy, Cell Culture
Figure Legend Snippet:
Techniques Used:
