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goat anti cd31  (R&D Systems)


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    Structured Review

    R&D Systems goat anti cd31
    Goat Anti Cd31, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+cd31/Human%2FMouse%2FRat+CD31%2FPECAM-1+Antibody/pmc13061539-116-12-16
    Average 98 stars, based on 1263 article reviews
    goat anti cd31 - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Immunostaining:

    Article Title: Galectin-3 mediates lysosome-related inflammation within monocyte-derived macrophages in a mouse model of ischemic brain injury
    Article Snippet: .. For immunostaining, we used goat anti-IBA1 (Abcam, ab5076), rabbit anti-NLRP3 (Abcam, ab263899), rabbit anti-CD11c (Abcam, ab219799), rat anti-GFAP (Invitrogen, 13-0300), rat anti-CD68 (Invitrogen, 14-0681-82), goat anti-CD31 (R&D Systems, AF3628), goat anti-MPO (R&D Systems, AF3667), mouse anti-GAL3 (Santa Cruz Biotechnology, sc-32790), rabbit anti-NEUN (Sigma-Aldrich, ABN78), rat anti-CD206 (Bio-Rad, MCA2235), and rabbit anti-MAP2 (Sigma-Aldrich, AB5622). .. For flow cytometry, we used CD11b-BUV737 (BD Biosciences, 612800), LY6G-BUV395 (BD Biosciences, 563978), CXCR4 BV510 (BD Biosciences, 563468), CD8 BV510 (BD Biosciences, 563068), CD11b-APC-Cy7 (BD Biosciences, 557657), CD11c BV421 (BD Biosciences, 562782), F4/80-BV605 (BD Biosciences, 743281), CD4 Pacific blue/eFluor 450 (Invitrogen, 48004182), CD11b-APC-eFluor 780 (Invitrogen, 47011282), CD45 PerCP-Cy5.5 (Invitrogen, 45045182), GAL3-PE (Invitrogen, 12530182), CD19 BV650 (BioLegend, 115541), NK1.1 BV785 (BioLegend, 108749), and NLRP3 APC (R&D Systems, IC7578A).

    Article Title: Impaired vitamin D signaling reveals neutrophils as key drivers of prostate cancer dissemination.
    Article Snippet: .. Immunostaining was performed by an overnight incubation at 4 °C with primary antibodies [rabbit antip-Akt Ser473 (Cell Signaling Technology Cat# 4060, RRID:AB_2315049; 1:200 dilution), rabbit anti-α-SMA (Cell Signaling Technology Cat# 19245, RRID:AB_2734735; 1:200 dilution), mouse anti-8-OHdG (Abcam Cat# ab48508, RRID:AB_867461; 1:100 dilution), rabbit anti-Ki-67 (Thermo Fisher Scientific Cat# MA5-14520, RRID:AB_10979488; 1:200 dilution), rabbit antiPanCK (Proteintech Cat# 26411-1-AP, RRID:AB_2880505; 1:500 dilution), rabbit anti-CD45 (Abcam Cat# ab10558, RRID:AB_442810; 1:600 dilution), goat anti-CD31 (R and D Systems Cat# AF3628, RRID:AB_2161028; 1:200 dilution), rabbit anti-F4/ 80 (Cell Signaling Technology Cat# 70076, RRID:AB_2799771; 1:200 dilution), rabbit anti-CD3 (Abcam Cat# ab16669, RRID:AB_443425; 1:200 dilution), rabbit anti-CK19 (Abcam Cat# ab133496, RRID:AB_11155282; 1:200 dilution)]. .. Ready to use antirabbit (Cell Signaling Technology Cat# 8114) or anti-mouse (Cell Signaling Technology Cat# 8125) Boost IHC Detection HRPconjugated secondary antibody was added for 30 min at RT.

    Article Title: Galectin-3 mediates lysosome-related inflammation within monocyte-derived macrophages in a mouse model of ischemic brain injury
    Article Snippet: .. For immunostaining: goat anti-IBA1 (Abcam, ab5076), rabbit anti-NLRP3 (Abcam, ab263899), rabbit anti-CD11c (Abcam, ab219799), rat anti-GFAP (Invitrogen, 13-0300), rat anti-CD68 (Invitrogen, 14-0681-82), goat anti-CD31 (R&D Systems, AF3628), goat anti-MPO (R&D Systems, AF3667), mouse anti-GAL3 (Santa Cruz Biotechnology, sc-32790), rabbit anti-NEUN (Sigma-Aldrich, ABN78), rat anti-CD206 (Bio-Rad, MCA2235), rabbit anti-MAP2 (Sigma-Aldrich, AB5622). .. For flow cytometry: CD11bBUV737 (BD Biosciences, 612800), LY6G-BUV395 (BD Biosciences, 563978), CXCR4 BV510 (BD Biosciences, 563468), CD8 BV510 (BD Biosciences, 563068), CD11b-APC-Cy7 (BD Biosciences, 557657), CD11c BV421 (BD Biosciences, 562782), F4/80-BV605 (BD Biosciences, 743281), CD4 Pacific Blue/eFlour 450 (Invitrogen, 48004182), CD11b-APC-efluor 780 (Invitrogen, 47011282), CD45 PerCP-Cy5.5 (Invitrogen, 45045182), GAL3-PE (Invitrogen, 12530182), CD19 BV650 (BioLegend, 115541), NK1.1 BV785 (BioLegend, 108749), NLRP3 APC (R&D systems, IC7578A).

    Incubation:

    Article Title: Impaired vitamin D signaling reveals neutrophils as key drivers of prostate cancer dissemination.
    Article Snippet: .. Immunostaining was performed by an overnight incubation at 4 °C with primary antibodies [rabbit antip-Akt Ser473 (Cell Signaling Technology Cat# 4060, RRID:AB_2315049; 1:200 dilution), rabbit anti-α-SMA (Cell Signaling Technology Cat# 19245, RRID:AB_2734735; 1:200 dilution), mouse anti-8-OHdG (Abcam Cat# ab48508, RRID:AB_867461; 1:100 dilution), rabbit anti-Ki-67 (Thermo Fisher Scientific Cat# MA5-14520, RRID:AB_10979488; 1:200 dilution), rabbit antiPanCK (Proteintech Cat# 26411-1-AP, RRID:AB_2880505; 1:500 dilution), rabbit anti-CD45 (Abcam Cat# ab10558, RRID:AB_442810; 1:600 dilution), goat anti-CD31 (R and D Systems Cat# AF3628, RRID:AB_2161028; 1:200 dilution), rabbit anti-F4/ 80 (Cell Signaling Technology Cat# 70076, RRID:AB_2799771; 1:200 dilution), rabbit anti-CD3 (Abcam Cat# ab16669, RRID:AB_443425; 1:200 dilution), rabbit anti-CK19 (Abcam Cat# ab133496, RRID:AB_11155282; 1:200 dilution)]. .. Ready to use antirabbit (Cell Signaling Technology Cat# 8114) or anti-mouse (Cell Signaling Technology Cat# 8125) Boost IHC Detection HRPconjugated secondary antibody was added for 30 min at RT.

    Article Title: Neutrophil Extracellular Traps Promote AIM2-Dependent Microglial Pyroptosis Following Stroke
    Article Snippet: .. Tissue sections were incubated with primary antibodies targeting key cellular markers, including goat anti-CD31 (1:200, AF3628; R&D Systems), rabbit anti-CitH3 (1:1 000, ab5103; Abcam), rat anti-Ly6G (1:200, 127602; BioLegend), rabbit anti-Iba1 (1:500, 10904-1-AP; Proteintech), mouse anti-Iba1 (1:200, RT1316; Huabio), rabbit anti-NeuN; 1:1 000, ab177487; Abcam), rabbit anti-GFAP, 1:500, 16825-1-AP; Proteintech), and mouse anti-AIM2 (1:1 000, sc-293174; Santa Cruz Biotechnology). .. Following primary antibody incubation, sections were treated with appropriate secondary antibodies, including Alexa Fluor 647-conjugated donkey anti-rabbit IgG (1:500, ab150075; Abcam), Alexa Fluor 488-conjugated donkey anti-mouse IgG (1:500, ab150153; Abcam), Cy3-conjugated donkey anti-goat IgG (1:100, SA00009-3; Proteintech), Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:500, ab150077; Abcam), and Alexa Fluor 594-conjugated goat anti-mouse IgG (1:500, ab150080; Abcam).

    Article Title: In vivo discovery of blood-brain barrier opening small molecules with FishNAP
    Article Snippet: .. Sagittal sections were blocked for 1 h at room temperature in PBS containing 10% normal donkey serum and 0.1% Triton X-100, then incubated overnight at 4°C with primary antibodies diluted in blocking buffer: goat anti-CD31 (1:100; R&D Systems AF3628), and rabbit anti-CLDN5 (1:100; Invitrogen 34-1600). .. Sections were subsequently washed in PBS and incubated for 2–3 h at room temperature with secondary antibodies (1:400; Jackson ImmunoResearch) and DyLight 405 Streptavidin (1:400; Jackson ImmunoResearch 016-470-084), then washed again in PBS and coverslipped.

    Microscopy:

    Article Title: Metabolite-induced DNA damage drives stochastic stem cell loss and clonal hematopoiesis
    Article Snippet: Cell suspensions from either source were spun unto slides using a Cytospin 4 (Thermo Scientific) and then stained using modified Wright stain (06689653 [Hematek Stain Pak] Hematek) on an automated staining platform (Hematek Bayer Health Care). .. Embryo whole-mount microscopy and analysis Whole-mount immunofluorescence on embryos was performed as previously described.38,96 Specifically, developmentally equivalent embryos (as assessed by somite count and vessel architecture) were stained using goat anti-CD31 (1:400; AF3628 R&D Systems followed by Alexa 488 donkey anti-goat IgG A1105 Invitrogen) and rat anti-C-KIT (1:200; 14-1171-85 eBioscience, followed by Donkey anti-Rat IgG (H+L) Alexa FluorTM 568; A78946 Invitrogen). .. Images were acquired on a Zeiss LSM 900 upright microscope using a Zeiss C-Achroplan 32x/0.85 W Corr objective.

    Immunofluorescence:

    Article Title: Metabolite-induced DNA damage drives stochastic stem cell loss and clonal hematopoiesis
    Article Snippet: Cell suspensions from either source were spun unto slides using a Cytospin 4 (Thermo Scientific) and then stained using modified Wright stain (06689653 [Hematek Stain Pak] Hematek) on an automated staining platform (Hematek Bayer Health Care). .. Embryo whole-mount microscopy and analysis Whole-mount immunofluorescence on embryos was performed as previously described.38,96 Specifically, developmentally equivalent embryos (as assessed by somite count and vessel architecture) were stained using goat anti-CD31 (1:400; AF3628 R&D Systems followed by Alexa 488 donkey anti-goat IgG A1105 Invitrogen) and rat anti-C-KIT (1:200; 14-1171-85 eBioscience, followed by Donkey anti-Rat IgG (H+L) Alexa FluorTM 568; A78946 Invitrogen). .. Images were acquired on a Zeiss LSM 900 upright microscope using a Zeiss C-Achroplan 32x/0.85 W Corr objective.

    Staining:

    Article Title: Metabolite-induced DNA damage drives stochastic stem cell loss and clonal hematopoiesis
    Article Snippet: Cell suspensions from either source were spun unto slides using a Cytospin 4 (Thermo Scientific) and then stained using modified Wright stain (06689653 [Hematek Stain Pak] Hematek) on an automated staining platform (Hematek Bayer Health Care). .. Embryo whole-mount microscopy and analysis Whole-mount immunofluorescence on embryos was performed as previously described.38,96 Specifically, developmentally equivalent embryos (as assessed by somite count and vessel architecture) were stained using goat anti-CD31 (1:400; AF3628 R&D Systems followed by Alexa 488 donkey anti-goat IgG A1105 Invitrogen) and rat anti-C-KIT (1:200; 14-1171-85 eBioscience, followed by Donkey anti-Rat IgG (H+L) Alexa FluorTM 568; A78946 Invitrogen). .. Images were acquired on a Zeiss LSM 900 upright microscope using a Zeiss C-Achroplan 32x/0.85 W Corr objective.

    Blocking Assay:

    Article Title: In vivo discovery of blood-brain barrier opening small molecules with FishNAP
    Article Snippet: .. Sagittal sections were blocked for 1 h at room temperature in PBS containing 10% normal donkey serum and 0.1% Triton X-100, then incubated overnight at 4°C with primary antibodies diluted in blocking buffer: goat anti-CD31 (1:100; R&D Systems AF3628), and rabbit anti-CLDN5 (1:100; Invitrogen 34-1600). .. Sections were subsequently washed in PBS and incubated for 2–3 h at room temperature with secondary antibodies (1:400; Jackson ImmunoResearch) and DyLight 405 Streptavidin (1:400; Jackson ImmunoResearch 016-470-084), then washed again in PBS and coverslipped.



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    Superior SA node myocytes exhibit elevated diastolic ATP and metabolic flux compared with the inferior region. (A) 3D segmented maximum-intensity projection of a whole-mount SA node immunolabeled for <t>CD31</t> (vasculature, red) and cyto-iATP (myocytes, green). The dashed line denotes the boundary between superior and inferior regions. (B) Image-processing workflow illustrating merged maximum-intensity projections, binary segmentation masks, and extraction of grayscale cyto-iATP signals used for quantitative analysis. (C) Mean cyto-iATP fluorescence intensity per myocyte, grouped by region ( N = 5 mice per region), reporting expression levels of the EGFP-tagged cyto-iATP sensor. (D) Live confocal imaging of cyto-iATP signals showing representative line-scan images and corresponding normalized fluorescence traces (F/F 0 ) from superior and inferior regions. (E and F) Summary quantification of cyto-iATP signal mass rate (E) and estimated diastolic [ATP] i (F). P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice.
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    Superior SA node myocytes exhibit elevated diastolic ATP and metabolic flux compared with the inferior region. (A) 3D segmented maximum-intensity projection of a whole-mount SA node immunolabeled for <t>CD31</t> (vasculature, red) and cyto-iATP (myocytes, green). The dashed line denotes the boundary between superior and inferior regions. (B) Image-processing workflow illustrating merged maximum-intensity projections, binary segmentation masks, and extraction of grayscale cyto-iATP signals used for quantitative analysis. (C) Mean cyto-iATP fluorescence intensity per myocyte, grouped by region ( N = 5 mice per region), reporting expression levels of the EGFP-tagged cyto-iATP sensor. (D) Live confocal imaging of cyto-iATP signals showing representative line-scan images and corresponding normalized fluorescence traces (F/F 0 ) from superior and inferior regions. (E and F) Summary quantification of cyto-iATP signal mass rate (E) and estimated diastolic [ATP] i (F). P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice.
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    Superior SA node myocytes exhibit elevated diastolic ATP and metabolic flux compared with the inferior region. (A) 3D segmented maximum-intensity projection of a whole-mount SA node immunolabeled for <t>CD31</t> (vasculature, red) and cyto-iATP (myocytes, green). The dashed line denotes the boundary between superior and inferior regions. (B) Image-processing workflow illustrating merged maximum-intensity projections, binary segmentation masks, and extraction of grayscale cyto-iATP signals used for quantitative analysis. (C) Mean cyto-iATP fluorescence intensity per myocyte, grouped by region ( N = 5 mice per region), reporting expression levels of the EGFP-tagged cyto-iATP sensor. (D) Live confocal imaging of cyto-iATP signals showing representative line-scan images and corresponding normalized fluorescence traces (F/F 0 ) from superior and inferior regions. (E and F) Summary quantification of cyto-iATP signal mass rate (E) and estimated diastolic [ATP] i (F). P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice.
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    Image Search Results


    Superior SA node myocytes exhibit elevated diastolic ATP and metabolic flux compared with the inferior region. (A) 3D segmented maximum-intensity projection of a whole-mount SA node immunolabeled for CD31 (vasculature, red) and cyto-iATP (myocytes, green). The dashed line denotes the boundary between superior and inferior regions. (B) Image-processing workflow illustrating merged maximum-intensity projections, binary segmentation masks, and extraction of grayscale cyto-iATP signals used for quantitative analysis. (C) Mean cyto-iATP fluorescence intensity per myocyte, grouped by region ( N = 5 mice per region), reporting expression levels of the EGFP-tagged cyto-iATP sensor. (D) Live confocal imaging of cyto-iATP signals showing representative line-scan images and corresponding normalized fluorescence traces (F/F 0 ) from superior and inferior regions. (E and F) Summary quantification of cyto-iATP signal mass rate (E) and estimated diastolic [ATP] i (F). P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice.

    Journal: The Journal of General Physiology

    Article Title: Beat-locked ATP microdomains in the sinoatrial node map a Ca 2+ -timed energetic hierarchy and regional pacemaker roles

    doi: 10.1085/jgp.202513874

    Figure Lengend Snippet: Superior SA node myocytes exhibit elevated diastolic ATP and metabolic flux compared with the inferior region. (A) 3D segmented maximum-intensity projection of a whole-mount SA node immunolabeled for CD31 (vasculature, red) and cyto-iATP (myocytes, green). The dashed line denotes the boundary between superior and inferior regions. (B) Image-processing workflow illustrating merged maximum-intensity projections, binary segmentation masks, and extraction of grayscale cyto-iATP signals used for quantitative analysis. (C) Mean cyto-iATP fluorescence intensity per myocyte, grouped by region ( N = 5 mice per region), reporting expression levels of the EGFP-tagged cyto-iATP sensor. (D) Live confocal imaging of cyto-iATP signals showing representative line-scan images and corresponding normalized fluorescence traces (F/F 0 ) from superior and inferior regions. (E and F) Summary quantification of cyto-iATP signal mass rate (E) and estimated diastolic [ATP] i (F). P values are shown above comparisons. Large circles denote per-animal means; small circles indicate individual biological replicates. N represents the number of independent mice.

    Article Snippet: For immunolabeling, SA nodes were incubated for 48 h at 4°C with a goat anti-mouse CD31 primary antibody (1:50, AF3628; R&D Systems).

    Techniques: Immunolabeling, Extraction, Fluorescence, Expressing, Imaging